Ethanol extracts of Scutellaria baicalensisprotect against lipopolysaccharide-induced acute liver injury in mice

http://repository.vnu.edu.vn/handle/VNU_123/11455

Objective: 
To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. 



Methods:
Dried roots of S. baicalensiswere extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. 
Animals were subsequently administered a single dose of LPS(5 mg/kg of body weight, intraperitoneal injection). 
Both protein and mRNAlevels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. 
Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting. 
Results:
Liver injury induced by LPSsignificantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. 
Treatment with ethanol extracts of S. baicalensisprevented all of these observed changes associated with LPS-induced injury in liver mice. 
Conclusions:
Our study showed that S. baicalensisis potentially protective against LPS-induced liver injury in mice


Title: Ethanol extracts of Scutellaria baicalensisprotect against lipopolysaccharide-induced acute liver injury in mice
Authors: Nguyen, Thanh Hai
Le, Tuan Anh
Duong, Thi Ly Huong
Nguyen, Huu Tung
Vu, Duc Loi
Dang, Thu Kim
Bui, Thanh Tung
Keywords: Scutellaria baicalensis;Lipopolysaccharide;Liver injury;Cytokine;Nuclear factor-κB
Issue Date: 2015
Publisher: Elsevier
Abstract: Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Methods:Dried roots of S. baicalensiswere extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS(5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNAlevels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting. Results:Liver injury induced by LPSsignificantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensisprevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions:Our study showed that S. baicalensisis potentially protective against LPS-induced liver injury in mice
URI: http://repository.vnu.edu.vn/handle/VNU_123/11455
ISSN: 2221-1691
Appears in Collections:SMP - Papers / Tham luận HN-HT

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